Confirming putative variants at ≤ 5% allele frequency using allele enrichment and Sanger sequencing
نویسندگان
چکیده
منابع مشابه
Evaluation of Allele Frequency Estimation Using Pooled Sequencing Data Simulation
Next-generation sequencing (NGS) technology has provided researchers with opportunities to study the genome in unprecedented detail. In particular, NGS is applied to disease association studies. Unlike genotyping chips, NGS is not limited to a fixed set of SNPs. Prices for NGS are now comparable to the SNP chip, although for large studies the cost can be substantial. Pooling techniques are ofte...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2021
ISSN: 2045-2322
DOI: 10.1038/s41598-021-91142-1